![]() However, a few steps into ImageJ, and this minor inconvenience is forgotten. One of the downsides of the Java heritage is an interface that may feel a little unfamiliar. The new 64-bit operating systems and their JRE have happily broken the long-held 1.7 Gb memory limit for Java applications. ![]() With JRE available for most operating systems, ImageJ is platform-independent, running on Macintosh, Windows, Linux, and even a PDA operating system. Java runtime environments (JRE) are freely available, either from Sun or bundled with platform-specific installations of ImageJ ( /ij). To run ImageJ, a given system needs only the operating system-specific Java runtime environment. ![]() By shifting to Java, Rasband liberated the software from an individual operating system. Wayne Rasband is the core author of ImageJ after developing the Macintosh-based National Institutes of Health (NIH) Image for 10 years, he made the brave decision of starting afresh with ImageJ using the Java programming language. In addition to its impressive functionality, this cutting-edge image-processing tool has an indispensable support community of enthusiasts on the ImageJ mailing list. These past 10 years have seen the Java-based open-source software mature into an invaluable laboratory tool. G.ImageJ will celebrate its tenth anniversary in September of this year. Legacy option for v0.2 and below: Adding new FEI/TFS microscope typesĭocumentation Changelog Other useful scalebar toolsĭM/GMS: Scale Bar Control by D. Restart Fiji and select the EMScaleBarTools from More Tools. It will add the FEI_Crop_Scalebar.ijm macro to the macros folder and the EMScaleBarTools.ijm toolset to the macros/toolset folder. Install via the Fiji update site.ĭownload the latest release, extract the macros folder, and copy it to your Fiji installation folder. □ Requirements and InstallationĬropping the FEI/TFS info bar requires the useful EM tool plugin by IMBalENce as FEI/TFS images are scaled with SEM FEI metadata scale. įor a description of the other tools, take a look at the wiki. ): In the batch processing menu insert the macro command runMacro('FEI_Crop_Scalebar.ijm'). Scale bar behaviour is the same as for QuickScaleBar tool and settings are taken from the QuickScaleBar options.Įspecially useful for batch conversion of SEM/FIB images (run from Process -> Batch -> Macro. One-click action to crop away the databar from an FEI/TFS SEM/FIB image and to add a scale bar. run('mpl-viridis') to change LUT to viridis. Optional: Run custom macro commands provided in the options menu, e.g. This is convenient for programs like PowerPoint which like to automatically interpolate "small" images. Optional: Automatically re-scale image to (at least) a specified image size in pixels without interpolation (= nearest neighbor interpolation). ![]() E.g., an image with horizontal field width of 0.25 µm will be switched to 250 nm. Optional: Automatically switch units to make scale bar more appealing. The scale bar appearance can be set up just like the normal settings for Analyze -> Tools -> Scale bar. The scale bar width is adjusted based on scaled image width (or height, or larger/smaller of the two) and rounded to next "good looking" number. The scale bar height and font size is adjusted based on image height (or width). One-click action to add a scale bar to an image. Macro description QuickScaleBar Tool (Icon: SB) The image are then copied via the hotkey c to the system clipboard and pasted into PowerPoint. Note the automatic handling of unit-switching and rescaling as in the previous example. For a horizontal alignment of images (here an SEM and an HAADF-STEM image) with the same desired image height, the scale bar reference is switched to Height. The next GIF shows an example workflow when working with presentations (here Microsoft PowerPoint). The next GIF shows the application of Auto re-scale images to upscale a small (in pixels) inset of an image: Note the similar size of the scale bars for the 4096² image (center) and the cropped 512² ROI image (right).īatch conversion of SEM images ( Process -> Batch -> Macro.) from tiff to png using FEI_Crop_Scalebar.ijm.īasic usage with cropping of a TFS/FEI databar, addition of a scale bar, moving and removing of the scale bar: Fiji macro toolset to quickly add a scale bar with reasonable size to an image.
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